Antibody Detection

BLASTOMYCES ANTIBODY IMMUNODIFFUSION

  • Test Code

    322

  • CPT Code

    86612

  • Clinical Significance

    The blastomyces antibody immunodiffusion test is used for the in vitro determination of precipitating antibodies to Blastomyces dermatitidis. It is also used as an aid in the diagnosis of Blastomycosis.

  • Methodology

    Fungal Immunodiffusion

  • Limitations

    The result is not intended to be used as the sole means for clinical diagnosis or patient management decisions.

    • Negative serologic tests may be observed among culturally demonstrable cases, which limits the predictive value of a negative test.
    • Negative serologic results obtained during the first month may warrant repeat testing.
    • Falsely negative results may be obtained when testing immunocompromised patients.
    • Test results cannot be used solely to distinguish between active infection and prior exposure.
  • Specimen Collection

    Serum: Collect serum specimens in serum separator or red top tube. Allow blood to clot for 30 minutes, then centrifuge. Pipette serum into a plastic screw cap vial.

  • Minimum Specimen Requirements

    Serum: 0.25 mL per antibody

  • Specimen Stability
    • Refrigerated: 14 days
    • Frozen: 6 months
  • Specimen Rejection

    Any specimen types other than serum.

  • Transport Temperature

    Refrigerated/Frozen

  • Shipping

    Ship on dry ice to arrive Monday‐Friday using a next day delivery service. Frozen ice packs may be substituted if sample is shipped the day of collection.

  • Turnaround Time

    Testing performed Tuesdays and Fridays.

    Serum: TAT 3 days from setup

  • Reference Range

    Antibody not detected

  • Interpretative Information
    • Negative: Antibody not detected
    • Positive: Antibody detected
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