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Antibody Detection

BLASTOMYCES IgG ANTIBODY EIA

  • Test Code

    331

  • CPT Code

    86612

  • Clinical Significance

    This assay indirectly detects anti-Blastomyces IgG by recognizing the BAD-1 repeat surface protein. This test offers an improvement in sensitivity over the current methods of detecting antibodies by immuno diffusion and complement fixation. The Blastomyces Antibody IgG EIA is useful for diagnosing blastomycosis in patients with negative antigen results. Secondly, when both Histoplasma and Blastomyces antigen assays are positive, comparing Histoplasma and Blastomyces antibody concentrations may assist in establishing the correct diagnosis. Additionally, following changes in antibody concentration via repeat testing may determine whether the infection has resolved or if relapse has occurred.

  • Methodology

    Semi-Quantitative Indirect Enzyme Immunoassay (EIA)

  • Limitations

    Specimens positive for Histoplasma capsulatum may cross-react with the Blastomyces Antibody EIA (IgG detection) and test positive (20% cross reactivity).

  • Specimen Collection
    • Serum: Serum separated from the clot in a leak-proof container.
    • Plasma: Sterile leak-proof container.
    • New York approved for Serum samples.
  • Minimum Specimen Requirements

    Serum/Plasma*: 0.25 mL

    * Plasma reported with rare specimen comment applied.

  • Specimen Stability
    • Room Temperature: 30 days
    • Refrigerated: 30 days
    • Frozen: 30 days
  • Specimen Rejection

    Any specimen type other than serum or plasma

  • Transport Temperature

    Ambient/Refrigerated/Frozen

  • Shipping

    Ship to arrive Monday‐Friday using a next day delivery service. Samples may be shipped on dry ice, frozen ice packs, or ambient.

  • Turnaround Time

    Same day**

    **Test Set up: Samples for Antigen testing received by 10:30AM will have same day test set up. Positive samples may require confirmation, which could extend turnaround time. Serum & CSF specimens are pre-treated to improve sensitivity, which can add one day to turnaround time.

  • Reference Range

    Negative

  • Interpretative Information
    • Negative: < 10.0 EU
    • Intermediate: 10.0 EU – 13.9 EU
    • Positive: 14.0 EU to ≥ 80.0 EU

    Results should be correlated with clinical presentation and history.

* New positives may require confirmation.

** Turnaround times are provided as ranges, and may vary depending on daily testing volumes and specimen type.

*** Test set up: Samples received by 10:30AM will have same-day test set up. Positive samples may require confirmation which may extend turnaround time. Serum & CSF specimens are pretreated to improve sensitivity which can add one day to turnaround time.

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