The coccidioides antibody immunodiffusion test is used for the in vitro determination of precipitating antibodies to Coccidioides TP and F antigens. It is also used as an aid in the diagnosis of Coccidioidomycosis.
The result is not intended to be used as the sole means for clinical diagnosis or patient management decisions.
Negative serologic tests may be observed among culturally demonstrable cases, which limits the predictive value of a negative test.
Negative serologic results obtained during the first month may warrant repeat testing.
Falsely negative results may be obtained when testing immunocompromised patients.
Test results cannot be used solely to distinguish between active infection and prior exposure.
Serum: Collect serum specimens in serum separator or red top tube. Allow blood to clot for 30 minutes, then centrifuge. Pipette serum into a plastic screw cap vial.
Minimum Specimen Requirements
Serum: 0.25 mL per antibody
Refrigerated: 14 days
Frozen: 6 months
Any specimen types other than serum.
Ship on dry ice to arrive Monday‐Friday using a next day delivery service. Frozen ice packs may be substituted if sample is shipped the day of collection.
Testing performed Tuesdays and Fridays.
Serum: TAT 3 days from setup
Antibody not detected
Will report F and TP specific
Negative: Antibody not detected
Positive: Antibody detected, will report titer up to ≥1:128
Antibodies to the TP antigen are typically detectable within 4 weeks of primary onset of infection,but are rarely detected 6 months after infection. The presence of antibodies to the TP antigen may suggest recent or active infection.
Antibodies to the F antigen are typically detectable by 6 weeks after the primary onset of the infection and may persist for several months to years.