The MVista® histoplasma quantitative antigen test aids the diagnosis of histoplasmosis. Monitoring the histoplasmosis helps determine when treatment can be stopped and to diagnose relapse.
Quantitative Sandwich Enzyme Immunoassay (EIA)
Serum and CSF are pre-treated to improve sensitivity
The result is not intended to be used as the sole means for the clinical diagnosis or patient management decisions.
Cross-reactions are seen with blastomycosis, paracoccidioidomycosis, penicilliosis, less frequently in coccidioidomycosis, rarely in aspergillosis and possibly sporotrichosis.
Sputolysin, sodium hydroxide and potassium hydroxide treatment degrade the analyte detected in the assay.
Serum: Collect serum specimens in serum separator or red top tube. Allow blood to clot for 30 minutes, then centrifuge. Pipette serum into a plastic screw cap vial.
Plasma: Collect plasma specimens in an EDTA, heparin or sodium citrate tube. Centrifuge for 15 minutes and pipette plasma into a plastic screw cap vial.
Urine/CSF/BAL/Other Body Fluid: Submit urine, CSF, BAL and all other body fluids in a sterile screw cap container.
Minimum Specimen Requirements
Serum/Plasma: 1.2 mL
CSF: 0.8 mL
Urine/ BAL/ Other Body Fluids: 0.5 mL
Room Temperature: 2 weeks
Refrigerated: 2 weeks
If specimen is too viscous to pipette
Tissue, Sputum, Bronchial Brushings, Stool, FNA, Biopsy, Tracheal or Bone Marrow Aspirate
Stored in Transport Media, Fixative or Isolator Tubes
Ship next day service for Monday-Friday delivery
Testing is performed Monday- Friday
Urine/BAL: Same day
Serum/Plasma/CSF: 1 day
*New positives may require confirmation
Positive Below the Limit of Quantification: Results above the cutoff for positivity, but below 0.4 ng/mL fall outside the linear range of the assay. These results are positive, but not accurately quantifiable.
Positive: 0.4-19.0 ng/mL
Positive Above the Limit of Quantification: Results greater than 19.0 ng/mL fall outside the linear range of the assay. These results are positive, but not accurately quantifiable.
MVista® is a registered trademark of MiraVista Diagnostics