ASPERGILLUS EIA GALACTOMANNAN TEST

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Various chronic Aspergillus pathologies may be found in immunocompetent subjects, and their diagnosis often proves difficult. A process of mold sensitization is at the root of allergic manifestations such as asthma, allergic broncho-pulmonary aspergillosis (ABPA), which represents the most exacerbated clinical form. Aspergilloma represents the most typical non-allergic chronic pathology that is the result of colonization of a preexistent pulmonary cavity by fungal mycelia forming an Aspergillus ball. The PlateliaTM Aspergillus IgG assay, used to detect class G immunoglobulins directed against a recombinant purified antigen of Aspergillus, represents a tool for diagnostic assistance of these chronic forms of aspergillosis that are observed in immunocompetent subjects.

• Platelia™ Aspergillus EIA immunoenzymatic sandwich microplate assay, cleared by the FDA for the detection of galactomannan in serum or BALF.
• Test validated at MiraVista Diagnostics for CSF specimens.

The result is not intended to be used as the sole means for clinical diagnosis or patient management decisions.

False positives can be caused by:

• Piperacillin-tazobactam, amoxicillin/clavulanic acid
• PLASMA-LYTE and other fluids containing sodium gluconate. Patients must be off PLASMA-LYTE for at least 3 days before collection.
• Infection with Penicillium, Alternaria, Paecilomyces, Fusarium Geotrichum, Histoplasma, and Blastomyces, or airway colonization with above organisms

*Antifungal therapy may cause false negative results in patients with aspergillosis
*Cross-reactions in CSF can occur with Histoplasma spp., Blastomyces spp., Cryptococcus neoformans, and Pseudomonas aeruginosa.

• Serum: Separated from clot in a leak-proof container.
• EDTA plasma: Separated from clot in a leak-proof container. (Reported with rare specimen comment applied.)
• CSF/BAL: Submit CSF and BAL in a sterile leak-proof container.
• New York approved for Serum, BAL, and CSF samples.

Serum/EDTA Plasma/CSF/BAL: 0.8 mL

CSF:

• Room Temperature: 30 days
• Refrigerated: 30 days
• Frozen: 60 days

Serum/BAL/EDTA Plasma:

• Room Temperature: 48 hours
• Refrigerated: 5 days
• Frozen: 5 months

• If specimen is too viscous to pipette.
• Nasal washings, plasma from any tube other than EDTA.
• Samples in fixative, transport media or PLASMA-LYTE.

Refrigerated/ Frozen

Ship on dry ice for next day service. Frozen ice packs may be substituted if sample is shipped the day of the collection. Monday – Friday delivery.

Serum/EDTA Plasma/CSF/BAL: Same Day

*New positives my require confirmation

**Test Set up: Samples for Antigen testing received by 10:30AM will have same day test set up. Positive samples may require confirmation which could extend turnaround time. Serum & CSF specimens are pretreated to improve sensitivity, which can add one day to turnaround time.

Negative

Negative: <0.5

Results are reported as numeric values (Index) which are interpreted as positive or negative

Platelia™ is a registered trademark of BioRad