COCCIDIOIDES QUANTITATIVE EIA TEST

• Test Code

  315

• CPT Code

  87449

• Clinical Significance

  The MVista® coccidioides quantitative antigen test aids in the diagnosis of coccidioidomycosis. Monitoring the coccidioidomycosis helps determine when treatment can be stopped and to diagnose relapse.

• Methodology
  • Quantitative Sandwich Enzyme Immunoassay (EIA)
  • Serum and CSF are pre-treated to improve accuracy
• Limitations

  The result is not intended to be used as the sole means for clinical diagnosis or patient management decisions.

  • Cross-reactions are seen with histoplasmosis, blastomycosis and paracoccidioidomycosis.
  • Sputolysin, sodium hydroxide, and potassium hydroxide treatment degrade the analyte detected in the assay.
• Specimen Collection
  • Serum: Collect serum specimens in serum separator or red top tube. Allow blood to clot for 30 minutes, then centrifuge. Pipette serum into a plastic screw cap vial.
  • Plasma: Collect plasma specimens in an EDTA, heparin or sodium citrate tube. Centrifuge for 15 minutes and pipette plasma into a plastic screw cap vial.
  • Urine/CSF/BAL/Other Body Fluids: Submit urine, CSF, BAL, and all other body fluids in a sterile screw cap container.
• Minimum Specimen Requirements
  • Serum/Plasma: 1.2 mL
  • CSF: 0.8 mL
  • Urine/BAL/Other Body Fluids: 0.5 mL
• Specimen Stability
  • Room Temperature: 14 days
  • Refrigerated: 14 days
  • Frozen: Indefinitely
• Specimen Rejection
  • If specimen is too viscous to pipette
  • Tissue, Sputum, Bronchial Brushings, Stool, FNA, Biopsy, Tracheal or Bone Marrow Aspirate
• Transport Temperature

  Refrigerated/Frozen/Room Temperature

• Shipping

  Ship next day service Monday- Friday delivery

   

• Turnaround Time

  Testing is performed Monday-Friday

  • Urine/BAL: Same day
  • Serum/Plasma/CSF: 1 day

  *New positives may require confirmation

   

• Reference Range

  None detected

• Interpretative Information
  • Positive: 0.07-8.2 ng/mL
  • Positive Above the Limit of Quantification: Results greater than 8.2 ng/mL fall outside the linear range of the assay. These results are positive, but not accurately quantifiable.
• Additional Information

  MVista® is a registered trademark of MiraVista Diagnostics