HISTOPLASMA QUANTITATIVE EIA TEST

• Test Code

  310

• CPT Code

  87385

• Clinical Significance

  The MVista® histoplasma quantitative antigen test aids the diagnosis of histoplasmosis. Monitoring the histoplasmosis helps determine when treatment can be stopped and to diagnose relapse.

• Methodology
  • Quantitative Sandwich Enzyme Immunoassay (EIA)
  • Serum and CSF are pre-treated to improve sensitivity
• Limitations

  The result is not intended to be used as the sole means for the clinical diagnosis or patient management decisions.

  • Cross-reactions are seen with blastomycosis, paracoccidioidomycosis, penicilliosis, less frequently in coccidioidomycosis, rarely in aspergillosis and possibly sporotrichosis.

  • Sputolysin, sodium hydroxide and potassium hydroxide treatment degrade the analyte detected in the assay.
• Specimen Collection
  • Serum:  Collect serum specimens in serum separator or red top tube. Allow blood to clot for 30 minutes, then centrifuge. Pipette serum into a plastic screw cap vial.
  • Plasma: Collect plasma specimens in an EDTA, heparin or sodium citrate tube. Centrifuge for 15 minutes and pipette plasma into a plastic screw cap vial.
  • Urine/CSF/BAL/Other Body Fluid: Submit urine, CSF, BAL and all other body fluids in a sterile screw cap container.
• Minimum Specimen Requirements
  • Serum/Plasma: 1.2 mL
  • CSF: 0.8 mL
  • Urine/ BAL/ Other Body Fluids: 0.5 mL
• Specimen Stability
  • Room Temperature: 2 weeks
  • Refrigerated: 2 weeks
  • Frozen: Indefinitely
• Specimen Rejection
  • If specimen is too viscous to pipette
  • Tissue, Sputum, Bronchial Brushings, Stool, FNA, Biopsy, Tracheal or Bone Marrow Aspirate
  • Stored in Transport Media, Fixative or Isolator Tubes
• Transport Temperature

  Refrigerated/Frozen/Room Temperature

• Shipping

  Ship next day service for Monday-Friday delivery

• Turnaround Time

  Testing is performed Monday- Friday

  • Urine/BAL: Same day
  • Serum/Plasma/CSF: 1 day

  *New positives may require confirmation

   

• Reference Range

  None detected

• Interpretative Information
  • Positive: 0.20-20.00 ng/mL
  • Positive Above the Limit of Quantification: Results greater than 20.00 ng/mL fall outside the linear range of the assay. These results are positive, but not accurately quantifiable.
• Additional Information

  MVista® is a registered trademark of MiraVista Diagnostics